SAP130/SF3B3 Antibody

SKU: orb18410

Description

Goat polyclonal antibody to SF3B3

Research Area

Epigenetics

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:2000, WB: 0.1-0.3 μg/ml
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:2000.WB: Approx 150kDa band observed in nuclear lysates of cell line HeLa (calculated MW of 136kDa according to NP_036558.3). Recommended concentration: 0.1-0.3 μg/ml.

Key Properties

• Clonality: Polyclonal
• Target: SAP130 / SF3B3
• Protein Sequence: VSKKLEDIRTRYAF
• Molecular Weight: 136
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti SF3B3 antibody, anti SAP130 antibody, anti splicing factor 3b, subunit 3, 130kDa antibody, anti RSE1 antibody, anti SF3B130 antibody, anti KIAA0017 antibody, anti STAF130 antibody, anti pre-mRNA splicing factor SF3b, 130 kDa subunit antibody, anti spliceosome-associated protein 130 antibody, anti splicing factor 3b, subunit 3 antibody, anti SF3b130 antibody

orb18410 staining (0.1ug/ml) of nuclear lysate from HeLa cells (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Quick Database Links

Gene Symbol

SAP130 / SF3B3

NCBI Gene ID

23450