SIRT4 Antibody

SKU: orb18632

Description

Goat polyclonal antibody to SIRT4

Research Area

Cancer, Cardiovascular Disease, DNA Damage

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:32000, WB: 1-3 μg/ml, IHC-P: 2-3 μg/ml
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Porcine, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Skeletal Muscle shows spotty staining of striated fibres in longitudinal section. Recommended concentration, 2-3ug/ml.WB: Approx. 38kDa band observed in Human Kidney and U937 lysates (calculated MW of 35.2kDa according to NP_036372.1). In transfected HEK293 transiently expressing SIRT4 a band of approx. 38kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 1-3 μg/ml.

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Key Properties

• Clonality: Polyclonal
• Target: SIRT4
• Protein Sequence: SRCGELLPLIDPC
• Molecular Weight: 35.2
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti SIRT4 antibody, anti SIR2L4 antibody, anti sirtuin (silent mating type information regulation 2 homolog) 4 (S. cerevisiae) antibody, anti sirtuin silent mating type information regulation 2 homolog 4 (S. cerevisiae) antibody, anti MGC130046 antibody, anti MGC130047 antibody, anti MGC57437 antibody, anti SIR2L4 antibody, anti sirtuin 4 antibody, anti sir2-like 4 antibody, anti sirtuin 4 antibody, anti sirtuin type 4 antibody

orb18632 Negative Control showing staining of paraffin embedded Human Cortex, with no primary antibody.

orb18632 (7µg/ml) staining of paraffin embedded Human Cortex. Heat induced antigen retrieval with citrate buffer Ph 6, HRP-staining.

orb18632 Negative Control showing staining of paraffin embedded Human Placenta, with no primary antibody.

orb18632 (7µg/ml) staining of paraffin embedded Human Placenta. Heat induced antigen retrieval with citrate buffer Ph 6, HRP-staining.

orb18632 Negative Control showing staining of paraffin embedded Human Kidney, with no primary antibody.

orb18632 (7µg/ml) staining of paraffin embedded Human Kidney. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

orb18632 (0.75ug/ml) staining of U937 (A), (2ug/ml) K562 (B) and (3ug/ml) U2OS (C), MCF7 (D), and U251 (E) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Quick Database Links

Gene Symbol

SIRT4

NCBI Gene ID

23409