Sirtuin 3 (mouse) Antibody

SKU: orb22501

Description

Goat Polyclonal to SIRT3

Research Area

Cardiovascular Disease

Images & Validation

• Tested Applications: ELISA, FC, IF, WB
• Dilution Range: ELISA: 1:64000, WB: 1-3 μg/ml, IHC-P: 10ug/ml
• Reactivity: Mouse
• Predicted Reactivity: Human, Porcine, Rat
• Application Notes:
  WB: Approx 30kDa band observed in Mouse Heart lysates (calculated MW of 28.8kDa according to NP_071878.2). Recommended concentration: 1-3 μg/ml. An additional band of unknown identity was also consistently observed at 15kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

  Read more...

Key Properties

• Clonality: Polyclonal
• Target: Sirtuin 3 (mouse)
• Protein Sequence: RASGIPASKLVEAH
• Molecular Weight: 28.8; 36.6
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti -SIR2-like protein 3 antibody, anti-OTTMUSP00000027445 antibody, anti-OTTMUSP00000027444 antibody, anti-OTTMUSP00000027441 antibody, anti-NAD-dependent deacetylase sirtuin-3 antibody, anti-Sir2l3 antibody, anti-AI848213 antibody, anti-2310003L23Rik antibody, anti-homolog) 3 antibody, anti-sirtuin 3 (silent mating type information regulation 2 antibody, anti-sirtuin 3 antibody, anti-Sirt3 antibody

orb22501 (0.3ug/ml) staining of Mouse Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunofluorescence analysis of NIH3T3 cells using SIRT3 antibody

Flow cytometric analysis of NIH3T3 cells using SIRT3 antibody

Quick Database Links

Gene Symbol

Sirtuin 3 (mouse)

NCBI Gene ID

23410