SMAD9 Antibody

SKU: orb19206

Description

Rabbit polyclonal antibody to SMAD9. The preotein is a member of the MAD-related family of molecules, and is thought to be essential components in the signaling pathways of the serine/threonine kinase receptors of the transforming growth factor beta superfamily. SMAD9 which regulate growth, differentiation, apoptosis, and development.

Research Area

Cancer, Epigenetics, Stem Cells

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:64000, WB: 0.3-1.0 μg/ml, IHC-P: 5 μg/ml
• Reactivity: Human
• Predicted Reactivity: Canine, Mouse, Rat
• Application Notes:
  This antibody is not expected to cross-react with SMAD1 and SMAD5.ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx 50kDa band observed in Human Skin lysates (calculated MW of 48.6kDa according to NP_005896.1). Recommended concentration: 0.3-1.0 μg/ml.

Key Properties

• Clonality: Polyclonal
• Target: SMAD9
• Protein Sequence: HSEPLMPHNATYPD
• Molecular Weight: 48.6
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti SMAD9 antibody, anti SMAD, mothers against DPP homolog 9 (Drosophila)] antibody, anti HGNC:6774 antibody, anti MADH6 antibody, anti MADH9 antibody, anti SMAD8A antibody, anti SMAD8B antibody, anti MAD (mothers against decapentaplegic, Drosophila) homolog 9 antibody, anti MAD, mothers against decapentaplegic homolog 9 antibody, anti MAD, mothers against decapentaplegic homolog 9 (Drosophila) antibody, anti Mothers against decapentaplegic, drosophila, homolog of, 9 antibody

Western blot analysis of Human Skin lysate using SMAD9 antibody

Immunohistochemical staining of Human Kidney using SMAD9 antibody.

Quick Database Links

Gene Symbol

SMAD9

NCBI Gene ID

4093