Smooth muscle alpha-actin Antibody

SKU: orb18863

Description

Goat polyclonal antibody to ACTA2

Research Area

Musculoskeletal & Connective Tissue Research

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:128000, WB: 0.3-1 μg/ml
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Canine
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:4000.IHC: Successful IHC-P data obtained from a previous batch. An anonymous customer found positive results in IHC-P on Pig heart tissue.WB: Approx 45kDa band observed in Human Duodenum lysates (calculated MW of 42.0kDa according to NP_001604.1). Recommended concentration: 0.1-0.3 μg/ml.Experiment Notes: Immunofluorescence: see publication McIntire RH et al., Placenta. 2008 Jul;29(7):631-8. Epub 2008 Jun 5. (PMID: 18538388).

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Key Properties

• Clonality: Polyclonal
• Target: Smooth muscle alpha-actin
• Protein Sequence: EEEDSTALVC
• Molecular Weight: 42
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Anti-a actin antibody, anti-AAT6 antibody, anti-ACTA_HUMAN antibody, anti-ACTA2 antibody, anti-Actin alpha 2 smooth muscle aorta antibody, anti-Actin aortic smooth muscle antibody, anti-Actin, aortic smooth muscle antibody, anti-ACTSA antibody, anti-ACTVS antibody, anti-Alpha 2 actin antibody, anti-Alpha actin 2 antibody, anti-Alpha cardiac actin antibody, anti-alpha sma antibody, anti-Alpha-actin-2 antibody

0.3 µg/ml staining of Human (A) and (0.5 ug/ml) Mouse (B) and Rat (C) Duodenum lysate (35 µg protein in RIPA buffer). Detected by chemiluminescence.

Quick Database Links

Gene Symbol

Smooth muscle alpha-actin

NCBI Gene ID

59

Nagy, Bence M. et al. Importance of kynurenine in pulmonary hypertension Am J Physiol Lung Cell Mol Physiol, 313, L741-L751 (2017)

PubMed: 28705908