SOD1 Antibody

SKU: orb19518

Description

Goat polyclonal antibody to SOD1

Research Area

Metabolism Research

Images & Validation

• Tested Applications: ELISA, IF, WB
• Dilution Range: ELISA: 1:8000, WB: 0.01-0.03 μg/ml, IHC-P: 10 μg/ml
• Reactivity: Human, Mouse
• Predicted Reactivity: Canine, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: Approx 18kDa band observed in Mouse Brain and Rat Spinal Cord lysates (calculated MW of 15.9kDa according to Human NP_000445.1, Mouse NP_035564.1 and Rat NP_058746.1). Recommended concentration: 0.01-0.03 μg/ml.

Key Properties

• Clonality: Polyclonal
• Target: SOD1
• Protein Sequence: SRKHGGPKDEERH
• Molecular Weight: 15.9
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti SOD1 antibody, anti superoxide dismutase 1, soluble (amyotrophic lateral sclerosis 1 (adult)) antibody, anti HGNC:11179 antibody, anti ALS antibody, anti ALS1 antibody, anti IPOA antibody, anti SOD antibody, anti Cu /Zn superoxide dismutase antibody, anti Cu/Zn superoxide dismutase antibody, anti SOD, soluble antibody, anti indophenoloxidase A antibody, anti superoxide dismutase (aa 120-154) antibody, anti superoxide dismutase 1, soluble antibody, anti superoxide dismutase, cystolic antibody

orb19518 (0.02ug/ml) staining of Mouse Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western blot analysis of NIH3T3 (Lane 1), HEK293 (Lane 2), HepG2 (Lane 3) and MCF7 (Lane 4) lysates using SOD1 antibody

Line graph illustrates about the Ag-Ab reactions using different concentrations of antigen and serial dilutions of using SOD1 antibody

Quick Database Links

Gene Symbol

SOD1

NCBI Gene ID

6647