SPFH2/ERLIN2 Antibody

SKU: orb19057

Description

Goat polyclonal antibody to ERLIN2

Research Area

Metabolism Research

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:2000, WB: 0.3-1 μg/ml, IHC-P: 3.75 μg/ml
• Reactivity: Human
• Predicted Reactivity: Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:2000.WB: Approx 43kDa band observed in Human Breast lysates and in lysates of Human Breast cancer (calculated MW of 37.8kDa according to NP_009106.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Pearce et al, J Biol Chem. 2007 Jul 13;282(28):20104-15.; PMID: 17502376). Recommended concentration: 0.3-1 μg/ml.

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Key Properties

• Clonality: Polyclonal
• Target: SPFH2 / ERLIN2
• Protein Sequence: EDEPLETATKEN
• Molecular Weight: 37.8
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti ERLIN2 antibody, anti SPFH2 antibody, anti ER lipid raft associated 2 antibody, anti SPFH domain family, member 2 antibody, anti HGNC:1356 antibody, anti C8orf2 antibody, anti MGC87072 antibody, anti chromosome 8 open reading frame 2 antibody, anti Erlin-2 antibody, anti MGC87072 antibody

orb19057 staining (1ug/ml) of Human Breast lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Immunohistochemical staining of Human Breast using ERLIN2 antibody

Quick Database Links

Gene Symbol

SPFH2 / ERLIN2

NCBI Gene ID

11160