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T-cell differentiation protein Antibody

SKU: orb19691

Description

Goat polyclonal antibody to CD8

Research Area

Stem Cell & Developmental Biology

Images & Validation

Tested ApplicationsELISA
Dilution RangeELISA: 1:4000, WB: 0.3 μg/ml
Predicted ReactivityHuman, Mouse, Rat
Application Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:4000.WB: Preliminary experiments gave an approx 35kDa band in Human Thymus and Thyroid Gland lysates after 0.3 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 16.7kDa according to NP_002362.1. The 35kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

ClonalityPolyclonal
TargetT-cell differentiation protein
Protein SequenceQDGFTYRHYHEN
Molecular Weight16.7, 12.1, 10.6
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

anti CD 8 antibody, anti CD 8A antibody, anti CD 8B antibody, anti CD8 antibody, anti CD8 antigen alpha polypeptide antibody, anti CD8 antigen, alpha polypeptide (p32) antibody, anti CD8A antibody, anti CD8a antigen antibody, anti CD8a molecule antibody, anti CD8A_HUMAN antibody, anti CD8B antibody, anti CD8b antigen antibody, anti CD8b antigen isoform 2 antibody, anti CD8b molecule antibody, anti CD8B protein antibody, anti CD8B1 antibody, anti Leu2 antibody, anti Leu2 T lymphocyte antigen antibody, anti Ly3 antibody, anti LYT3 antibody, anti MAL antibody, anti MGC119115 antibody, anti OKT8 T Cell antigen antibody, anti p32 antibody, anti T cell antigen Leu2 antibody, anti T cell co receptor antibody, anti T cell surface glycoprotein CD8 alpha chain antibody, anti T cell surface glycoprotein CD8 beta chain antibody, anti T lymphocyte differentiation antigen T8/Leu 2 antibody, anti T-cell surface glycoprotein CD8 alpha chain antibody, anti T-lymphocyte differentiation antigen T8/Leu-2 antibody, anti T8 T cell antigen antibody

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NCBI Gene Details

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Protocol Information

ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

T-cell differentiation protein Antibody (orb19691)

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100 μg
$ 360.00
Free Secondary Antibody (20 ul)0/0

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