UBE2L3 Antibody

SKU: orb18828

Description

Goat polyclonal antibody to UBE2L3

Images & Validation

• Tested Applications: ELISA, IF, WB
• Dilution Range: ELISA: 1:128000, WB: 1-3 μg/ml, IHC-P: 5 μg/ml
• Reactivity: Human, Mouse, Porcine, Rat
• Predicted Reactivity: Bovine
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.WB: Approx 18kDa band observed in Hela and Jurkat lysates (predicted MW of 18kDa according to NP_003338). Recommended for use at 0.5-2 μg/ml.

Key Properties

• Clonality: Polyclonal
• Target: UBE2L3
• Protein Sequence: EFTKKYGEKRPVD
• Molecular Weight: 17.9
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti UBE2L3 antibody, anti ubiquitin-conjugating enzyme E2L 3 antibody, anti UBCH7 antibody, anti E2-F1 antibody, anti L-UBC antibody, anti UbcM4 antibody, anti ubiquitin carrier protein antibody, anti ubiquitin-conjugating enzyme UBCH7 antibody, anti ubiquitin-protein ligase antibody

EB05770 (0.01µg/ml) staining of HEK293 (A), HepG2 (B) and Jurkat (C) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

EB05770 (0.03µg/ml) staining of Human (A), Mouse (B) and Rat (C) Skeletal Muscle lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunofluorescence staining of MCF7 (A) and Hela (B) cells with 5ug/ml EB05770 antibody. Detected with Rabbit anti-goat IgG-Alexafluor488 antibody at 1:1000. Nuclei Counterstained with DAPI.

EB05770 (0.01µg/ml) staining of Human (A), Mouse (B), Rat (C) and Pig (D) Heart lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

EB05770 staining (0.5µg/ml) of Hela lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Quick Database Links

Gene Symbol

UBE2L3

NCBI Gene ID

7332