ZASP/ CYPHER/LDB3 Antibody

SKU: orb19099

Description

Goat polyclonal antibody to LDB3

Research Area

Cardiovascular Disease, Epigenetics

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:1000, WB: 0.1-1 μg/ml
• Reactivity: Human
• Predicted Reactivity: Canine, Mouse, Porcine, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:2000.WB: Experiments gave bands at approx 80-90kDa and 30-35kDa in Human Skeletal Muscle lysates after 0.01 μg/ml antibody staining. These bands correspond to earlier findings in literature with different antibodies (Huang et al J Biol Chem. 2003 Feb 28;278(9):7360-5; PMID: 12499364). This protein has a calculated MW of 77.1kDa according to NP_009009.1. Recommended concentration: 0.01-0.03 μg/ml.

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Key Properties

• Clonality: Polyclonal
• Target: ZASP/ CYPHER / LDB3
• Protein Sequence: KSQNKPEDEADE
• Molecular Weight: 77.1; 66.7; 35.6; 31.0
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti LDB3 antibody, anti ZASP antibody, anti LIM domain binding 3 antibody, anti HGNC:15710 antibody, anti KIAA01613 antibody, anti KIAA0613 antibody, anti ORACLE antibody, anti PDLIM6 antibody, anti PDZ and LIM domain 6 antibody, anti Z-band alternatively spliced PDZ-motif antibody, anti CYPHER antibody, anti FLJ35865 antibody, anti ldb3z1 antibody, anti ldb3z4 antibody

Western blot analysis of human muscle lysate using LDB3 antibody

Immunohistochemical staining of Human Skeletal Muscle using LDB3 antibody

Quick Database Links

Gene Symbol

ZASP/ CYPHER / LDB3

NCBI Gene ID

11155