Goat IgG (H&L) Secondary Antibody

SKU: orb346980

Description

GOAT IgG (H&L) antibody

Research Area

Cell Biology

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:50,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:10,000 - 1:60,000
• Reactivity: Goat
• Application Notes:
  Anti-Goat IgG antibody has been tested by ELISA, SDS-PAGE, and western blot, and is suitable for immunohistochemistry, as well as other assays requiring lot-to-lot consistency.

Key Properties

• Antibody Type: Secondary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Goat IgG whole molecule
• Purity: Secondary Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Goat IgG and Goat Serum.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 2.1 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

rabbit anti-GOAT IgG Antibody, rabbit anti goat IgG

Western Blot of Rabbit anti-Goat IgG Antibody. Lane 1: Goat IgG (p/n orb2652767). Load: 50 ng per lane. Primary antibody: Goat antibody at 1:1000 overnight at 4°C. Secondary antibody: DyLight™ 649 rabbit secondary antibody at 1:20000 for 30 min at RT. Block: orb348637 for 30 min at RT. Predicted/Observed size: 28 & 55 kDa, 28 & 55 kDa for Goat IgG. Other band(s): None