Grem1 Antibody

SKU: orb1263984

Description

Grem1 Antibody

Research Area

Signal Transduction

Images & Validation

• Tested Applications: IHC-P, WB
• Reactivity: Human, Mouse
• Predicted Reactivity: Rat
• Application Notes:
  For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Immunogen: This GREMLIN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 147-175 amino acids from the C-terminal region of mouse GREMLIN.
• Target: Grem1
• Molecular Weight: 21 kDa
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Gremlin-1, Cysteine knot superfamily 1, BMP antagonist 1, Down-regulated in Mos-transformed cells protein, Grem1, Cktsf1b1, Drm

Western blot analysis of anti-Gremlin Pab in CEM cell line lysates (35 ug/lane)

Western blot analysis of anti-Gremlin Pab in mouse liver cell lysate. Gremlin was detected by using AP6133a.

Formalin-PBS and Bouin embedded paraffin human renal tissue pretreated with heat induced epitope retrieval Citrate buffer pH 6.0 in microwave. Dilution of primary antibody was 1:50. Detection method was ABC system or Vectastaint Elite VECTOR.

Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with GREMLIN Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Quick Database Links

Gene Symbol

Grem1

UniProt

O70326

RefSeq:O70326