GW182 Rabbit Polyclonal Antibody

SKU: orb183979

Description

GW182 Rabbit Polyclonal Antibody

Research Area

Epigenetics and Nuclear Signaling

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P
• Dilution Range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Rat
• Predicted Reactivity: Human, Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human GW182 (631-730/1962aa)
• Target: TNRC6A
• Molecular Weight: 210 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CAGH26; FAME6; GW1; GW182; TNRC6; 2010321I05Rik; 3110054G10Rik; D130023A07Rik; TNR6A_HUMAN; TNRC6A; CAG repeat protein 26; EMSY interactor protein; GW182 autoantigen (Protein GW1); Glycine-tryptophan protein of 182 kDa; KIAA1460; TNR6A_MOUSE; trinucleotide repeat containing adaptor 6A; trinucleotide repeat containing 6; trinucleotide repeat containing 6A

Paraformaldehyde-fixed, paraffin embedded Rat Stomach, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with GW182 Polyclonal Antibody, Unconjugated (orb183979) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Quick Database Links

Gene Symbol

TNRC6A

UniProt

Q8NDV7

Dittmann, Klaus et al. New roles for nuclear EGFR in regulating the stability and translation of mRNAs associated with VEGF signaling PLoS One, 12, e0189087 (2017)

PubMed: 29253018

Applications

WB

Reactivity

Human