HEC1 Rabbit Polyclonal Antibody

SKU: orb101439

Description

HEC1 Rabbit Polyclonal Antibody

Research Area

Cancer Research, Cell Biology, Cell Cycle

Images & Validation

• Tested Applications: WB
• Dilution Range: WB=1:500-2000
• Reactivity: Mouse
• Predicted Reactivity: Bovine, Canine, Equine, Human, Porcine, Rabbit, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human HEC1 (21-120/642aa)
• Target: NDC80
• Molecular Weight: 74 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

HEC; HEC1; HsHec1; KNTC2; TID3; hsNDC80; 2610020P18Rik; NDC80_HUMAN; NDC80; Highly expressed in cancer protein; Kinetochore protein Hec1 (HsHec1); Kinetochore-associated protein 2; Retinoblastoma-associated protein HEC; NDC80_MOUSE; Kinetochore protein Hec1; NDC80 kinetochore complex component; highly expressed in cancer, rich in leucine heptad repeats (yeast); kinetochore associated 2; NDC80 kinetochore complex component homolog (S. cerevisiae)

Sample: Testis (Mouse) Lysate at 40 ug, Primary: Anti-HEC1 (orb101439) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 74 kD, Observed band size: 74 kD.

Sample: Thymus (Mouse) Lysate at 40 ug, Primary: Anti-HEC1 (orb101439) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 74 kD, Observed band size: 83 kD.

Quick Database Links

Gene Symbol

NDC80

UniProt

O14777