NEDD9 Antibody

SKU: orb344432

Description

HEF1 antibody

Research Area

Cancer Biology

Images & Validation

• Tested Applications: ELISA, IF, IP, WB
• Dilution Range: ELISA: 1:5,000 - 1:20,000, IF: 1:100, IP: 1:100, WB: 1:500
• Reactivity: Human
• Application Notes:
  This monoclonal antibody has been tested for use in western blotting, immunoprecipitation and immunofluorescence. This clone recognizes HEF1 under non-denaturing conditions. Specific conditions for reactivity should be optimized by the end user. Expect bands approximately 115 and 105 in size corresponding to isoforms of HEF1 protein by western blotting in the appropriate cell lysate or extract. This antibody does not recognize p130Cas. Sin1 has not been tested. IF was performed using 4% PFA fixed cells. This monoclonal mostly detects HEF1 localized at the focal adhesion sites.

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Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone No.: 14A11
• Immunogen: Anti-HEF1 monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to amino acid residues 82-398 of human HEF1 protein.
• Target: NEDD9
• Purity: This Protein A purified antibody is directed against human HEF1 protein. The product was purified from tissue culture supernatant by chromatography. This antibody has only been tested on human cells. Reactivity against multiple isoforms is expected. Reactivity against homologues from other sources is not known. Specificity was determined by partial epitope mapping.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

mouse anti-hEF1 antibody, mouse anti-NEDD-9 antibody, mouse anti-CASL antibody, Cas like docking antibody, CASL antibody, Crk associated substrate related protein antibody, dJ49G10.2 antibody, dJ761I2.1 antibody, Enhancer of filamentation 1 antibody

Immunofluorescence microscopy using Biorbyt's Monoclonal anti-HEF1 antibody (clone 14A11) shows detection of HEF1 localized at the centrosome (bright dots) and focal adhesion sites. The antibody was used at a 1:100 dilution with a 1-min exposure time.

Western blotting using Biorbyt's Monoclonal anti-HEF1 antibody (clone 14A11) shows detection of HEF1 present in MCF-7 cells induced to express HEF1 by tetracycline removal (right lane).

Quick Database Links

Gene Symbol

NEDD9

UniProt

Q14511

RefSeq:5453680