ErbB2 Antibody / HER2

SKU: orb248331

Description

This antibody recognizes a protein of 185kDa, which is identified as HER2 / ErbB2. Its epitope is localized in the extracellular domain. ErbB2 is a member of the EGFR family. This antibody is specific and shows minimal cross-reaction with other members of the EGFR-family. Receptors of this family are located on the plasma membrane and consist of an extracellular ligand-binding domain that is connected to a large intracellular domain by a single transmembrane sequence. ErbB2 protein is over-expressed in a variety of carcinomas especially those of breast and ovary.

Research Area

Signal Transduction

Images & Validation

• Tested Applications: ELISA, FACS, IF
• Dilution Range: ELISA (order BSA/sodium azide-free format for coating),Flow cytometry: 1-2ug/10^6 cells,Immunofluorescence: 1-3ug/ml
• Reactivity: Human
• Application Notes:
  Variations in protocols, secondaries and substrates may require the antibody to be titered for optimal performance.1. This ErbB2 antibody binds to the extracellular/cell surface region of the protein.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2a, kappa
• Clone No.: HRB2/258
• Immunogen: Recombinant human protein was used as the immunogen for this ErbB2 antibody.(1)
• Purification: Protein G purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

SDS-PAGE Analysis of Purified, BSA-Free ErbB2 Antibody (clone HRB2/258). Confirmation of Integrity and Purity of the Antibody.