Histone H1.2 Recombinant Rabbit Monoclonal Antibody

SKU: orb783428

Description

Histone H1.2 Recombinant Rabbit Monoclonal Antibody

Research Area

Epigenetics & Chromatin

Images & Validation

• Tested Applications: FC, ICC, WB
• Dilution Range: WB=1:500-2000, ICC/IF=1:50-200, Flow-Cyt=1:50-100
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: IgG
• Clone No.: B1E1
• Immunogen: KLH conjugated synthetic peptide derived from human Histone H1.2
• Target: H1-2
• Molecular Weight: 32 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

H1.2; H1C; H1F2; H1s-1; HIST1H1C; H12_HUMAN; H1-2; Histone H1c; Histone H1d; Histone H1s-1; H1.2 linker histone, cluster member; H1 histone family, member 2; histone 1, H1c; histone cluster 1, H1c; histone cluster 1 H1 family member c

Paraformaldehyde-fixed, paraffin embedded (human kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Histone H1.2) Monoclonal Antibody, Unconjugated (orb783428) at 1:50 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Histone H1.2) Monoclonal Antibody, Unconjugated (orb783428) at 1:50 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse colon), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Histone H1.2) Monoclonal Antibody, Unconjugated (orb783428) at 1:50 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Histone H1.2) Monoclonal Antibody, Unconjugated (orb783428) at 1:50 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

PC-3M cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (Histone H1.2) monoclonal Antibody, Unconjugated (orb783428) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Sample: Lane 1: Hela cell lysate, Lane 2: 293 cell lysate, Lane 3: MCF-7 cell lysate, Primary: Anti-Histone H1.2 (orb783428) at 1:500 dilution, Secondary: Goat Anti-Rabbit IgG - HRP at 1:5000 dilution, Predicted band size: 21 kD, Observed band size: 25 kD.

Sample: Lane 1: rat liver tissue lysate, Lane 2: mouse lung tissue lysate, Primary: Anti-Histone H1.2 (orb783428) at 1:500 dilution, Secondary: Goat Anti-Rabbit IgG - HRP at 1:5000 dilution, Predicted band size: 21 kD, Observed band size: 25 kD.

SK-Br-3 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (Histone H1.2) monoclonal Antibody, Unconjugated (orb783428) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Quick Database Links

Gene Symbol

H1-2

UniProt

P16403