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Description
Research Area
Epigenetics & Chromatin
Images & Validation
−
| Tested Applications | ELISA, FC, ICC, IF, IHC, IHC-Fr, WB |
|---|---|
| Dilution Range | Western Blot: 1/500 - 1/2000. IHC: 1/100 - 1/300. ICC/IF: 1/200 - 1/1000. ELISA: 1/10000. IHC-f,Flow Cyt 1:500 |
| Reactivity | Drosophila, Human, Mouse, Rat |
Key Properties
−| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Histone H3.1 around the phosphorylation site of Ser10. AA range:1-50 |
| Molecular Weight | 15 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Concentration | 1 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Anti-HIST1H3A antibody, anti-H3FA antibody, anti-HIST1H3B antibody, anti-H3FL antibody, anti-HIST1H3C antibody, anti-H3FC antibody, anti-HIST1H3D antibody, anti-H3FB antibody, anti-HIST1H3E antibody, anti-H3FD antibody, anti-HIST1H3F antibody, anti-H3FI antibody, anti-HIST1H3G antibody, anti-H3FH antibody, anti-HIST1H3H antibody, anti-H3FK antibody, anti-HIST1H3I antibody, anti-H3FF antibody, anti-HIST1H3J antibody, anti-H3FJ antibody, anti-Histone H3.1 antibody, anti-Histone H3/a antibody, anti-Histone H3/b antibody

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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IHC-Fr
Immunohistochemistry Frozen
FC
Flow Cytometry
IF
Immunofluorescence
ICC
Immunocytochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
Histone H3 (phospho Ser10) rabbit pAb Antibody (orb764202)
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