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Description
Research Area
Cancer Biology, Epigenetics & Chromatin
Images & Validation
−Item 1 of 3
| Tested Applications | IF, IHC, WB |
|---|---|
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human HMGA1 (NP_665908.1). |
| Target | HMGA1 |
| Molecular Weight | Observed: 20kDa |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
| Concentration | batch dependent |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−nonhistone chromosomal high-mobility group protein HMG-I/HMG-Y, high-mobility group (nonhistone chromosomal) protein isoforms I and Y, OTTHUMP00000039618, OTTHUMP00000016224, OTTHUMP00000016223, OTTHUMP00000016222, MGC4854, MGC4242, MGC12816, HMGIY, HMGA1A, HMG-R, high mobility group AT-hook 1, HMGA1
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Western blot analysis of extracts of 293T cells, using HMGA1 antibody (orb1259044) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human stomach using HMGA1 antibody (orb1259044) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using HMGA1 antibody (orb1259044) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Quick Database Links
Gene Symbol
HMGA1
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
HMGA1 Antibody (orb1259044)
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