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HMGB1 Antibody

SKU: orb248159

Description

HMGB1 is a promoter of the immune response to injury or infection. It is released from dying cells (necrotic, not apoptotic), activating the immune system in anticipation of infection and encouraging tissue repair by attracting and promoting the proliferation of stem cells.

Images & Validation

Tested ApplicationsWB
Dilution RangeWestern blot: 2-4ug/ml
ReactivityHuman, Mouse
Application Notes
Provided assay concentrations are suggestions only, HMGB1 antibody titration may be required for optimal results.

Key Properties

Antibody TypePrimary Antibody
HostMouse
ClonalityMonoclonal
IsotypeMouse IgG1, kappa
Clone No.HMG314
ImmunogenRecombinant human HMGB1 was used as the immunogen for this antibody.
PurificationProtein G Affinity Chromatography
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives0.5 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin, 0.05% sodium azide
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

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HMGB1 Antibody

Western blot testing of human samples with HMGB1 antibody HMG314 at 2 ug/ml. Predicted molecular weight ~25 kDa.

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol

HMGB1 Antibody (orb248159)

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Available Sizes

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25 μg
$ 330.00
100 μg
$ 600.00
Free Secondary Antibody (20 ul)0/0

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