HNRNPU Antibody

SKU: orb1257795

Description

HNRNPU Antibody

Research Area

Epigenetics & Chromatin

Images & Validation

• Tested Applications: IF, WB
• Reactivity: Human, Rat
• Application Notes:
  WB: 1:500 - 1:2000IF: 1:50 - 1:200

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 608-825 of human HNRNPU (NP_114032.2).
• Target: HNRNPU
• Molecular Weight: Observed: 120kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

EIEE54, GRIP120-AS1, HNRPU, SAF-A, SAFA, U21.1, hnRNP U, pp12HNRNPU, heterogeneous nuclear ribonucleoprotein U, HNRNPU antisense RNA 1, heterogeneous nuclear ribonucleoprotein U (scaffold attachment factor A), nuclear p120 ribonucleoprotein, p120 nuclear protein

Western blot analysis of extracts of various cell lines, using HNRNPU antibody (orb1257795) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunofluorescence analysis of C6 cells using HNRNPU antibody (orb1257795) at dilution of 1:100. Blue: DAPI for nuclear staining.

Quick Database Links

Gene Symbol

HNRNPU

UniProt

Q00839