HSP27 Rabbit Polyclonal Antibody

SKU: orb500777

Description

HSP27 Rabbit Polyclonal Antibody

Research Area

Actina, Cancer Research, Cardiovascular Research, Signal Transduction, Tumor Biomarkers

Images & Validation

• Tested Applications: FC, IF, IHC-Fr, IHC-P
• Dilution Range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=3ug/Test
• Reactivity: Human, Rat
• Predicted Reactivity: Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human HSP27 (101-170/205aa)
• Target: HSPB1
• Molecular Weight: 27 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CMT2F; HEL-S-102; HMN2B; HMND3; HS.76067; HSP27; HSP28; Hsp25; SRP27; 27kDa; HSPB1_CANLF; HSPB1; Heat shock 27 kDa protein (HSP 27); HSPB1_HUMAN; 28 kDa heat shock protein; Estrogen-regulated 24 kDa protein; Heat shock protein family B member 1; Stress-responsive protein 27 (SRP27); HSPB1_MOUSE; Growth-related 25 kDa protein; Heat shock 25 kDa protein (HSP 25); p25; HSPB1_RAT; heat shock protein family B (small) member 1; heat shock 27kD protein 1; heat shock 27kDa protein 1

Blank control (blue line): A431 cells (fixed with 70% methanol (Overnight at 4°C) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C). Primary Antibody (green line): Rabbit Anti-HSP27 antibody (orb500777), dilution: 3 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, dilution: 1 µg/Test.

Paraformaldehyde-fixed, paraffin embedded (Rat stomach), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (HSP27) Polyclonal Antibody, Unconjugated (orb500777) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat uterus), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (HSP27) Polyclonal Antibody, Unconjugated (orb500777) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Quick Database Links

Gene Symbol

HSPB1

UniProt

P04792