HSP60/GROEL Antibody

SKU: orb1151529

Description

Rabbit monoclonal antibody to HSP60

Research Area

Neuroscience

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
• Reactivity: Human, Mouse, Rat
• Application Notes:
  Optimal dilution of the HSP60/GROEL antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: Rabbit IgG, kappa
• Clone No.: HSPD1/6498R
• Immunogen: Recombinant human full-length HSP60 protein was used as the immunogen for the HSP60/GROEL antibody. Its epitope is localized between amino acids 383-447 of human hsp60.
• Purification: Protein A/G affinity
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin, 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Western blot testing of human 1) Hep2 and 2) HeLa cell lysate using HSP60/GROEL antibody (clone HSPD1/6498R). Predicted molecular weight: ~60 kDa.

Western blot testing of human Hep2 cell lysate using HSP60/GROEL antibody (clone HSPD1/6498R). Predicted molecular weight: ~60 kDa.

IHC staining of FFPE human liver tissue with HSP60/GROEL antibody (clone HSPD1/6498R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Quick Database Links

UniProt

P10809