HSP90B1 Rabbit Polyclonal Antibody

SKU: orb627523

Description

Rabbit polyclonal antibody to GRP94

Research Area

Cell Biology

Images & Validation

• Tested Applications: ELISA, FC, IF, IHC, IP, WB
• Dilution Range: WB: 1:1000-1:4000; IP: 1:500-1:2000; IHC: 1:20-1:200; IF: 1:20-1:200
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: heat shock protein 90kDa beta(Grp94), member 1
• Target: HSP90B1
• Molecular Weight: 94 kDa
• Purity: ≥95% as determined by SDS-PAGE
• Purification: Immunogen affinity purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: liquid
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ECGP, Endoplasmin, GP96, gp96 homolog, GRP 94, GRP94, HSP90B1, TRA1, Tumor rejection antigen 1

A549 cells were subjected to SDS PAGE followed by western blot with orb627523 (HSP90B1 antibody) at dilution of 1:1000.

Immunofluorescent analysis of HepG2 cells, using HSP90B1 antibody orb627523 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

IP Result of anti-GRP94 (IP: orb627523, 3 ug; Detection: orb627523 1:1000) with mouse liver tissue lysate 4000 ug.

Immunohistochemistry of paraffin-embedded human breast cancer using orb627523 (HSP90B1 antibody) at dilution of 1:50.

Quick Database Links

Gene Symbol

HSP90B1

UniProt

P14625