Human ATG3 protein

SKU: orb391267

Description

Recombinant human ATG3 protein is produced by our E. coli expression system and the target gene encoding Met1-His287 is expressed with a T7 tag at the N-terminus, 6His tag at the C-terminus.

Research Area

Cell Biology

Images & Validation

• Tested Applications: ELISA, MS, SDS-PAGE, WB
• Application Notes:
  Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Key Properties

• Source: E. coli
• Target: ATG3
• Reactivity: Human
• Endotoxins: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
• Purity: Greater than 95% as determined by reducing SDS-PAGE.
• Protein Sequence: MASMTGGQQM GRGSMQNVIN TVKGKALEVA EYLTPVLKES KFKETGVITP EEFVAAGDHL VHHCPTWQWA TGEELKVKAY LPTGKQFLVT KNVPCYKRCK QMEYSDELEA IIEEDDGDGG WVDTYHNTGI TGITEAVKEI TLENKDNIRL QDCSALCEEE EDEDEGEAAD MEEYEESGLL ETDEATLDTR KIVEACKAKT DAGGEDAILQ TRTYDLYITY DKYYQTPRLW LFGYDEQRQP LTVEHMYEDI SQDHVKKTVT IENHPHLPPP PMCSVHPCRH AEVMKKIIET VAEGGGELGV HLEHHHHHH

Storage & Handling

• Storage: Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.2 μM filtered solution of 20mM Tris-HCl, 150mM NaCl, 10% Glycerol, pH 8.0.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

APG3; APG3L; Ubiquitin-like-conjugating enzyme ATG3; Autophagy-related protein 3; APG3-like; hApg3; Protein PC3-96

Quick Database Links

Gene Symbol

ATG3

UniProt

Q9NT62

NCBI Gene ID

64422