Human Dkk-1 Protein, His Tag

SKU: orb257395

Description

Human Dkk-1 Protein, His Tag, premium grade is expressed from human 293 cells (HEK293). It contains AA Thr 32 - His 266 (Accession # NP_036374.1).

Research Area

Stem Cell & Developmental Biology

Images & Validation

• Application Notes:
  This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 26.6 kDa. The protein migrates as 35-45 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Key Properties

• Expression System: HEK293
• Target: Dkk-1
• Reactivity: Human
• Tag: C-6×His
• Expression Region: Thr 32 - His 266
• Endotoxins: 0.1 EU per μg
• Conjugation: Unconjugated
• MW: 26.6 kDa
• Purity: 95%

Storage & Handling

• Storage: -20°C
• Form/Appearance: Powder
• Buffer/Preservatives: PBS, pH 7.4
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

SDS-PAGE analysis of Human Dkk-1 protein

Immobilized Human Dkk-1 Protein, His Tag, premium grade (Cat. No. orb257395) at 5 μg/mL (100 μL/well) can bind Human LRP-5, Mouse IgG2a Fc Tag (Cat. No. orb570288) with a linear range of 0.01-0.156 μg/mL (QC tested).

Quick Database Links

Gene Symbol

Dkk-1

UniProt

NP_036374.1

RefSeq (Protein):NP_036374.1

Li, Chunyan et al. The Effect of C-X-C Motif Chemokine 13 on Hepatocellular Carcinoma Associates with Wnt Signaling Biomed Res Int, 2015, 345413 (2015)

PubMed: 26161394