Human IGF-I R Protein, Fc Tag

SKU: orb1496214

Description

The Insulin-like Growth Factor 1 Receptor (IGF1) is also known as CD221, JTK13. and is a transmembrane receptor that is activated by IGF-1 and by the related growth factor IGF-2. It belongs to the large class of tyrosine kinase receptors. This receptor mediates the effects of IGF-1, which is a polypeptide protein hormone similar in molecular structure to insulin. IGF1R is make up of two alpha subunits and two beta subunits, the Both the α and β subunits are synthesized from a single mR precursor. The precursor is then glycosylated, proteolytically cleaved, and crosslinked by cysteine bonds to form a functional transmembrane αβ chain.The α chains are located extracellularly while the β subunit spans the membrane and are responsible for intracellular signal transduction upon ligand stimulation. IGF1R have a binding site for ATP, which is used to provide the phosphates for autophosphorylation. There is a 60% homology between IGF1R and the insulin receptor. In response to ligand binding, the α chains induce the tyrosine autophosphorylation of the β chains. This event triggers a cascade of intracellular signaling that, while somewhat cell type specific, often promotes cell survival and cell proliferation.

Research Area

Disease Biomarkers

Images & Validation

• Application Notes:
  This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 129.3 kDa. The protein migrates as 70-75 kDa and 105-115 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Key Properties

• Expression System: HEK293
• Target: IGF-I R / IGF-1 R
• Reactivity: Human
• Tag: C-Fc
• Expression Region: Glu 31 - Asn 932
• Endotoxins: 1.0 EU per μg
• Conjugation: Unconjugated
• MW: 129.3 kDa
• Purity: 95%

Storage & Handling

• Storage: -20°C
• Form/Appearance: Powder
• Buffer/Preservatives: PBS, pH 7.4
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Immobilized Human IGF-I R, Fc Tag (Cat. No. orb1496214) at 5 μg/mL (100 μL/well) can bind Biotinylated Human IGF-I, Avitag, Fc Tag with a linear range of 0.01-0.625 μg/mL (QC tested).

Quick Database Links

Gene Symbol

IGF-I R / IGF-1 R

UniProt

P08069

RefSeq:P08069