Human IgG (H&L) Antibody Rhodamine Conjugated

SKU: orb347259

Description

Human IgG (H&L) antibody (TRITC)

Images & Validation

• Tested Applications: DOT, IF
• Dilution Range: IF: 1:500-1:2,500
• Reactivity: Human
• Application Notes:
  Mouse Anti-Human IgG Rhodamine conjugated has been tested by dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Key Properties

• Antibody Type: Secondary Antibody
• Host: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Human IgG whole molecule
• Purity: Human IgG (H&L) Antibody Rhodamine Conjugated was prepared from monospecific polyclonal ascites by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum, Human IgG and Human Serum.
• Conjugation: TRITC

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

mouse anti-Human IgG rhodamine conjugated Antibody, mouse anti-Human IgG Antibody TRITC conjugation

Dot Blot of Mouse Anti-Human IgG Rhodamine Conjugated Secondary Antibody. Antigen: Human IgG. Load: 3-fold serial dilution starting at 200 ng. Primary antibody: None. Secondary antibody: Rhodamine mouse secondary antibody at 1:1000 for 1H at RT. Block: orb348637 for 1 H at RT.