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Description
Research Area
Images & Validation
−| Application Notes |
|---|
Key Properties
−| Expression System | HEK293 |
|---|---|
| Target | IL-12B |
| Reactivity | Human |
| Tag | C-10×His |
| Expression Region | Ile 23 - Ser 328 |
| Endotoxins | 1.0 EU per μg |
| Conjugation | Unconjugated |
| MW | 36.6 kDa |
| Purity | 95% |
Storage & Handling
−| Storage | -20°C |
|---|---|
| Form/Appearance | Powder |
| Buffer/Preservatives | PBS, pH 7.4 |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
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−Human IL12B Protein, His Tag [orb1290821]
Unconjugated
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 35.5 kDa after removal of the signal peptide. The apparent molecular mass of IL12B-His is approximately 35-55 kDa due to glycosylation.
Mammalian
Human IL 23 Protein (His Tag) [orb168630]
Greater than 95.0% as determined by analysis by SDS-PAGE.
Sf9, Baculovirus cells

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SDS-PAGE analysis of Human IL-12B protein

Loaded Human IL-12 R beta 1, Fc Tag (Cat. No. orb570282) on Protein A Biosensor, can bind Human IL-12B, His Tag (Cat. No. orb257709) with an affinity constant of 3.78 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
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Human IL-12B Protein, His Tag (orb257709)
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