Human UCHL3 Protein

SKU: orb429104

Description

Recombinant of human UCHL3 protein. Ubiquitin carboxyl-terminal hydrolase isozyme L3 belongs to a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to produce the ubiquitin monomer. UCHL3 takes part in the regulation of neuronal development and spermatogenesis and is associated to neurodegenerative diseases. UCHL3 has a 54% homology to UCHL1.

Images & Validation

• Application Notes:
  Enzymes

Key Properties

• Source: Escherichia Coli
• Biological Activity: Specific activity: >3,000 pmole/min/ug. Measured by the hydrolysis of Ubiquitin-AMC at pH 8.0, at 37C
• Immunogen: non-glycosylated polypeptide chain containing 250 amino acids (1-230aa)
• Tag: 20 amino acid His-tag at N-terminus
• MW: 28.3kDa
• Purity: > 95% as determined by SDS-PAGE
• Protein Sequence: MGSSHHHHHH SSGLVPRGSH MEGQRWLPLE ANPEVTNQFL KQLGLHPNWQ FVDVYGMDPE LLSMVPRPVC AVLLLFPITE KYEVFRTEEE EKIKSQGQDV TSSVYFMKQT ISNACGTIGL IHAIANNKDK MHFESGSTLK KFLEESVSMS PEERARYLEN YDAIRVTHET SAHEGQTEAP SIDEKVDLHF IALVHVDGHL YELDGRKPFP INHGETSDET LLEDAIEVCK KFMERDPDEL RFNAIALSAA

Storage & Handling

• Storage: Store at 4°C if the entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage, it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid freeze thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 20mM Tris-HCl buffer (pH8.0), 1mM DTT, and 10% glycerol.
• Concentration: 1mg/ml
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Ubiquitin Carboxyl-Terminal Esterase L3 (ubiquitin thiolesterase), UCH-L3, Ubiquitin Carboxyl-Terminal Hydrolase Isozyme L3, EC 3.4.19.12