NFKBIA Antibody

SKU: orb1274539

Description

NFKBIA Antibody

Research Area

Cancer Biology

Images & Validation

• Tested Applications: IP, KO/KD Validated, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB: 1:500 - 1:2000IP: 1:20 - 1:50

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-317 of human IKB alpha (NP_065390.1).
• Target: NFKBIA
• Molecular Weight: Observed: 36kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

NFKBIA, IKBA, MAD-3, NFKBI

Western blot analysis of extracts of various cell lines, using IKB alpha antibody (orb1274539) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Western blot analysis of extracts from normal (control) and IKB alpha knockout (KO) 293T cells, using IKB alpha antibody (orb1274539) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Immunoprecipitation analysis of 150 ug extracts of A549 cells using 3 ug IKB alpha antibody (orb1274539). Western blot was performed from the immunoprecipitate using IKB alpha antibody (orb1274539) at a dilution of 1:500.

Quick Database Links

Gene Symbol

NFKBIA

UniProt

P25963