IKK Alpha + IKK beta Rabbit Polyclonal Antibody

SKU: orb157675

Description

IKK Alpha + IKK beta Rabbit Polyclonal Antibody

Research Area

Signal Transduction

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P
• Dilution Range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Porcine, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human IKK Alpha/IKK beta (151-250/756aa)
• Target: CHUK
• Molecular Weight: 83 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

BPS2; IKBKA; IKK-1; IKK-alpha; IKK1; IKKA; NFKBIKA; TCF16; IKKA_HUMAN; CHUK; I-kappa-B kinase alpha; IKK-A; IkappaB kinase; Conserved helix-loop-helix ubiquitous kinase; I-kappa-B kinase 1 (IKK-1 | IKK1); Nuclear factor NF-kappa-B inhibitor kinase alpha (NFKBIKA); Transcription factor 16 (TCF-16); 2.7.11.10; IKKA_MOUSE; I-kappa-B kinase 1 (IKK1); component of inhibitor of nuclear factor kappa B kinase complex; inhibitor of nuclear factor kappa-B kinase subunit alpha; I-kappa-B kinase

Paraformaldehyde-fixed, paraffin embedded (human liver), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (IKK Alpha + IKK beta) Polyclonal Antibody, Unconjugated (orb157675) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (IKK Alpha + IKK beta) Polyclonal Antibody, Unconjugated (orb157675) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Quick Database Links

Gene Symbol

CHUK

UniProt

O15111

Bennett, Marissa et al. Proteoglycan 4 Reduces Neuroinflammation and Protects the Blood-Brain Barrier after Traumatic Brain Injury J Neurotrauma, 38, 385-398 (2021)

PubMed: 32940130