IKK gamma Rabbit Polyclonal Antibody

SKU: orb608081

Description

IKK gamma Rabbit Polyclonal Antibody

Research Area

Cancer Research, Inflammation, Inflammatory Mediators, Kinases, NF-kB Signaling Pathway, NF-kB Signalling Pathway, TLR Signaling Pathway

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human
• Predicted Reactivity: Bovine, Mouse, Porcine, Rat, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human IKK gamma (301-400/419aa)
• Target: IKBKG
• Molecular Weight: 50 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

AMCBX1; EDAID1; FIP-3; FIP3; Fip3p; IKK-gamma; IKKAP1; IKKG; IMD33; IP; IP1; IP2; IPD2; NEMO; SAIDX; ZC2HC9; 1110037D23Rik; IKK[g]; NEMO_HUMAN; IKBKG; IkB kinase-associated protein 1 (IKKAP1); Inhibitor of nuclear factor kappa-B kinase subunit gamma (I-kappa-B kinase subunit gamma | IKK-gamma | IKKG | IkB kinase subunit gamma); NF-kappa-B essential modifier; NEMO_MOUSE; IkB kinase-associated protein 1 (IKKAP1 | mFIP-3); NEMO_RAT; inhibitor of nuclear factor kappa B kinase regulatory subunit gamma; incontinentia pigmenti; inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase gamma; inhibitor of nuclear factor kappa B kinase subunit gamma; NF-kappa-B essential modulator; IkB kinase-associated protein 1; I-kappa-B kinase subunit gamma; IkB kinase subunit gamma; 14.7K (adenovirus E3 protein) interacting protein 3

Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (IKK gamma) Polyclonal Antibody, Unconjugated (orb608081) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human pancreatic cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (IKK gamma) Polyclonal Antibody, Unconjugated (orb608081) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Hela (Human) Cell Lysate at 30 ug, Jurkat (Human) Cell Lysate at 30 ug, K562 (Human) Cell Lysate at 30 ug, Primary: Anti-IKK gamma (orb608081) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 50 kD, Observed band size: 50 kD.

Quick Database Links

Gene Symbol

IKBKG

UniProt

Q9Y6K9