IL-23 Rabbit Polyclonal Antibody

SKU: orb10907

Description

IL-23 Rabbit Polyclonal Antibody

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: ELISA, IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
• Reactivity: Human, Mouse
• Predicted Reactivity: Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human IL-23A
• Target: Il23a
• Molecular Weight: 21 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

IL-23; IL-23A; IL23P19; P19; SGRF; IL23A_HUMAN; IL23A; IL-23 subunit alpha; IL-23-A; Interleukin-23 subunit p19 (IL-23p19); IL23A_MOUSE; IL23A_RAT; interleukin 23 subunit alpha; interleukin 23, alpha subunit p19; interleukin-six, G-CSF related factor

Sample: Lane 1: Mouse Ear tissue lysates, Lane 2: Human A431 cell lysates, Lane 3: Human Jurkat cell lysates, Primary: Anti-IL-23 (orb10907) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 21 kDa, Observed band size: 21 kDa.

Tissue/Cell: human rhinopolypus tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-IL-23 Polyclonal Antibody, Unconjugated (orb10907) 1:300, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

Il23a

UniProt

Q9NPF7

A Wojtkowiak-Giera 1, D Kosik-Bogacka 2, N Łanocha-Arendarczyk 3, A Kolasa 4, K Kot 2, P Solarczyk 1, M Derda Toll-like receptors and inflammatory cytokines in the skin of Acanthamoeba spp. infected immunocompetent and immunosuppressed mice Exp Parasitol ., (2025)

PubMed: 40274043

Applications

IHC

Reactivity

Mouse

Mohamed A. El-Sayed et al. Muscone alleviated acetic acid-induced ulcerative colitis in mice through inhibiting NF-κB, IL-23/IL-17A axis, COX-2, and oxidative stress Beni-Suef University Journal of Basic and Applied Sciences, (2026)

Applications

IHC

Reactivity

Mouse

Mohamed A et al. Potential therapeutic effects of escin in mitigating inflammation and oxidative stress in experimentally induced ulcerative colitis in mice by acetic acid The Journal of Basic and Applied Zoology, (2026)

Applications

IHC

Reactivity

Mouse