Integrin alpha 6 Recombinant Rabbit Monoclonal Antibody

SKU: orb704187

Description

Integrin alpha 6 Recombinant Rabbit Monoclonal Antibody

Research Area

Basal Lamina, Cell Biology, Cell Signaling, Intercellular Signaling, Molecular Biology, Stem Cells, Surface Molecules

Images & Validation

• Tested Applications: WB
• Dilution Range: WB=1:500-2000
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: IgG
• Clone No.: B6H9
• Immunogen: A synthesized peptide derived from human Integrin alpha 6 (1-56/1130aa)
• Target: ITGA6
• Molecular Weight: 130-140 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CD49f; ITGA6A; ITGA6B; JEB6; VLA-6; 5033401O05Rik; ITA6_HUMAN; ITGA6; CD49 antigen-like family member F; ITA6_MOUSE; integrin subunit alpha 6; integrin, alpha 6; very late activation protein 6

Paraformaldehyde-fixed, paraffin embedded (human tonsil), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Integrin alpha 6) Monoclonal Antibody, Unconjugated (orb704187) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Lung (Mouse) Lysate at 40 ug, Lane 2: Spleen (Mouse) Lysate at 40 ug, Primary: Anti-Integrin alpha 6 (orb704187) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 120 kD, Observed band size: 120 kD.

Quick Database Links

Gene Symbol

ITGA6

UniProt

P23229