Cxcl10 Antibody

SKU: orb1272655

Description

Cxcl10 Antibody

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: ELISA, NeA, WB
• Reactivity: Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Produced from sera of rabbits immunized with highly pure recombinant Human Heregulin- β1. Human Heregulin- β1 specific antibody was purified by affinity chromatography and then biotinylated.
• Target: Cxcl10
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

IP-10, Scyb10, Inp10, Mob1, C-X-C motif chemokine 10, 10 kDa interferon gamma-induced protein, Gamma-IP10

To detect Rat IP-10 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Rat IP-10 (orb1272652) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IP-10.

To detect Rat IP-10 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat IP-10 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

To detect Rat IP-10 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat IP-10 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

This antibody stained colchicine injected rat brain (including the caudate putamen) tissue. The primary antibody was incubated at 0.25 ug/ml overnight at 4 °C. This was followed by a peroxidase conjugated secondary antibody and then a fluorescein Tyramide Signal Amplification (TSA) reagent. Optimal concentrations and conditions may vary.

Quick Database Links

Gene Symbol

Cxcl10

UniProt

P48973

RefSeq:P48973