IRE1 Rabbit Polyclonal Antibody

SKU: orb526774

Description

IRE1 Rabbit Polyclonal Antibody

Research Area

Kinases

Images & Validation

• Tested Applications: FC, WB
• Dilution Range: WB=1:500-2000, Flow-Cyt=1:100-200
• Reactivity: Human, Porcine
• Predicted Reactivity: Human, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human IRE1 (19-120/977aa)
• Target: ERN1
• Molecular Weight: 120 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

IRE1-BETA; IRE1b; IRE2p; hIRE2p; Ern1; Ire1; mIre1; ERN1_HUMAN; Endoplasmic reticulum-to-nucleus signaling 1; Inositol-requiring protein 1 (hIRE1p); Ire1-alpha (IRE1a); ERN1_MOUSE; Inositol-requiring protein 1; endoplasmic reticulum to nucleus signaling 1; ER to nucleus signalling 1; inositol-requiring enzyme 1; Serine/threonine-protein kinase/endoribonuclease IRE1

Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (IRE1) Polyclonal Antibody, Unconjugated (orb526774) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Pancreas (Mouse) Lysate at 40 ug, Primary: Anti-IRE1 (orb526774) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 105 kD, Observed band size: 105 kD.

Sample: Pig, Primary: Anti-IRE1 (orb526774) at 1/500 dilution.

Quick Database Links

Gene Symbol

ERN1

UniProt

O75460