FGF7 Antibody

SKU: orb1272420

Description

FGF7 Antibody

Research Area

Signal Transduction

Images & Validation

• Tested Applications: ELISA, WB
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hKGF (human Keratinocyte Growth Factor).
• Target: FGF7
• Purification: Anti-hKGF specific antibody was purified by affinity chromatography employing immobilized hKGF matrix.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

KGF, HBGF-7, KGF, Fibroblast growth factor 7, Heparin-binding growth factor 7, FGF-7

To detect hKGF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Anti-Human KGF (orb1272419) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hKGF.

To detect hKGF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hKGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

To detect hKGF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hKGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

This antibody stained formalin-fixed, paraffin-embedded sections of normal human skin. The recommended concentration is 0.5 ug/ml overnight at 4°C. An HRP-labeled polymer detection system was used with a non-alcohol soluble AEC chromogen and a proteinase K antigen retrieval. Optimal concentrations and conditions may vary.

Quick Database Links

Gene Symbol

FGF7

UniProt

P21781

RefSeq:P21781