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Description
Research Area
Images & Validation
−| Tested Applications | IHC, WB |
|---|---|
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 20-300 of human KLKB1 (NP_000883.2). |
| Target | KLKB1 |
| Molecular Weight | Observed: 71kDa |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
| Concentration | batch dependent |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−KLKB1 Rabbit Polyclonal Antibody [orb13526]
ELISA, IHC-P, WB
Human, Mouse, Porcine, Rat
Rabbit
Polyclonal
Unconjugated
Human Pyruvate Kinase, Liver and RBC (PK) ELISA Kit [orb776064]
Human
1.57-100 ng/mL
0.6 ng/mL
Human Kallikrein 3 (KLK3) ELISA Kit [orb775062]
Human
0.2-12.5 ng/mL
0.08 ng/mL
Rat Kallikrein 3 (KLK3) ELISA Kit [orb775344]
Rat
0.16-10 ng/mL
0.055 ng/mL
Mouse Kallikrein 3 (KLK3) ELISA Kit [orb775345]
Mouse
15.63-1000 pg/mL
6.4 pg/mL

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Western blot analysis of extracts of various cell lines, using KLKB1 antibody (orb1257560) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Immunohistochemistry of paraffin-embedded rat kidney using KLKB1 antibody (orb1257560) at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded rat brain using KLKB1 antibody (orb1257560) at dilution of 1:200 (40x lens).
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Protocol Information
KLKB1 Antibody (orb1257560)
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