LPO Antibody

SKU: orb750615

Description

LPO antibody

Research Area

Metabolism Research

Images & Validation

• Tested Applications: ELISA, IP, WB
• Dilution Range: ELISA: 1:18,000 - 1:75,000, IP: 1:100, WB: 1:2,000 - 1:10,000
• Reactivity: Bovine
• Application Notes:
  Anti-Lactoperoxidase has been tested in western blot and is suitable in ELISA and immunoprecipitation. Specific conditions for reactivity should be optimized by the end user.

Key Properties

• Antibody Type: Primary Antibody
• Host: Sheep
• Clonality: Polyclonal
• Isotype: Antiserum
• Immunogen: Lactoperoxidase [Bovine Milk]
• Target: LPO
• Purity: This product was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-sheep serum, purified and partially purified Lactoperoxidase [Bovine Milk]. Cross reactivity against Lactoperoxidase from other tissues and species may occur but have not been specifically determined.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Concentration: 90 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

sheep anti-Lactoperoxidase Antibody, Lactoperoxidase, LPO

Biorbyt Sheep anti Lactoperoxidase antibody was used to detect Lactoperoxidase under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of ~1 ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody (ON 4 C in orb348637). Detection shown was using Dylight 488 conjugated secondary antibody (1:10K in TBS/orb348637 1 hr RT).

Quick Database Links

Gene Symbol

LPO

UniProt

P80025

RefSeq:P80025.1