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LAMC2 Antibody

SKU: orb1258414

Description

LAMC2 Antibody

Research Area

Cell Biology, Neuroscience, Signal Transduction

Images & Validation

Tested ApplicationsIF, WB
ReactivityHuman, Mouse, Rat
Application Notes
WB: 1:500 - 1:2000IF: 1:50 - 1:100

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 862-1111 of human LAMC2 (NP_061486.2).
TargetLAMC2
Molecular WeightObserved: 131kDa
PurificationAffinity purification
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/PreservativesPBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Concentrationbatch dependent
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

Laminin subunit gamma-2, Cell-scattering factor 140 kDa subunit, CSF 140 kDa subunit, Epiligrin subunit gamma, Kalinin subunit gamma, Kalinin/nicein/epiligrin 100 kDa subunit, Ladsin 140 kDa subunit, Laminin B2t chain, Laminin-5 subunit gamma, Large adhesive scatter factor 140 kDa subunit, Nicein subunit gamma, LAMC2, LAMB2T, LAMNB2

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LAMC2 Antibody

Western blot analysis of extracts of rat lung, using LAMC2 antibody (orb1258414) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

LAMC2 Antibody

Immunofluorescence analysis of A431 cells using LAMC2 Polyclonal Antibody (orb1258414) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

LAMC2 Antibody

Immunofluorescence analysis of rat kidney using LAMC2 Polyclonal Antibody (orb1258414) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

LAMC2 Antibody

Immunofluorescence analysis of mouse kidney using LAMC2 Polyclonal Antibody (orb1258414) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

UniProt Details

No UniProt data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IF
Immunofluorescence
View Protocol

LAMC2 Antibody (orb1258414)

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Available Sizes

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100 μl
$ 830.00
Free Secondary Antibody (20 ul)0/0

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