LIAS Rabbit Polyclonal Antibody

SKU: orb184921

Description

LIAS Rabbit Polyclonal Antibody

Research Area

Cell Biology, Cell Markers, Mitochondrial, Mitochondrial Markers

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution Range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, WB=1:500-2000
• Reactivity: Mouse
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Human, Porcine, Rabbit, Rat, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human LIAS (28-130/372aa)
• Target: LIAS
• Molecular Weight: 39 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

HGCLAS; HUSSY-01; LAS; LIP1; LS; PDHLD; 2900022L22Rik; 4933425M12Rik; 7a5ex; lip-syn; zgc:66080; LIAS_BOVIN; LIAS; Lipoate synthase (LS | Lip-syn); Lipoic acid synthase; 2.8.1.8; LIAS_HUMAN; LIAS_MOUSE; Lipoate synthase (LS | Lip-syn | mLIP1); LIAS_RAT; lipoic acid synthetase; Lipoyl synthase, mitochondrial

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (LIAS) Polyclonal Antibody, Unconjugated (orb184921) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Quick Database Links

Gene Symbol

LIAS

UniProt

O43766