LIPA/Lysosomal Acid Lipase Antibody

SKU: orb1535659

Description

LIPA/Lysosomal Acid Lipase Antibody

Research Area

Metabolism Research

Images & Validation

• Tested Applications: ELISA, IHC, IHC-P, IP, WB
• Dilution Range: ELISA, IHC, IHC-P (10 µg/ml), IP, WB
• Reactivity: Human
• Application Notes:
  Further information: Sandwich ELISA: Recombinant protein. Western Blot: Transfected lysate, Recombinant protein.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2b,k
• Clone No.: 1F9
• Immunogen: LIPA (AAH12287, aa 1-399). A full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Target: LIPA / Lysosomal Acid Lipase
• Purification: Purified from ascites by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS, pH 7.4
• Concentration: 0.5 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

LIPA, Cholesterol ester hydrolase, Cholesteryl esterase, LAL, Lysosomal acid lipase, Lipase A, Sterol esterase, CESD

Western Blot analysis of LIPA expression in transfected 293T cell line by LIPA monoclonal antibody (M01), clone 1F9.Lane 1: LIPA transfected lysate (45 KDa).Lane 2: Non-transfected lysate.

Immunoprecipitation of LIPA transfected lysate using anti-LIPA monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with LIPA rabbit polyclonal antibody.

Detection limit for recombinant GST tagged LIPA is approximately 10 ng/ml as a capture antibody.

Quick Database Links

Gene Symbol

LIPA / Lysosomal Acid Lipase