LSD1 Antibody / Lysine-Specific Demethylase 1

SKU: orb248156

Description

LSD1 (Lysine-specific demethylase 1), also called KDM1 (lysine (K)-specific demethylase 1A), is the first histone demethylase to be identified and regulates transcription via demethylation of lysines 4 and 9 of Histone H3. It negatively regulates pro-inflammatory cytokine expression by interacting with the genes promoter region. It also plays a role in tumor development where overexpression of LSD1 correlates with a poor prognosis. It is upregulated in cancers of the bladder, breast, lung, colon, and GI tract as well as neuroblastoma and cells of mesenchymal origin such as chondrosarcoma, osteosarcoma, rhabdomyosarcoma and Ewing's sarcoma (Bennani-Baiti et al, 2012).

Research Area

Metabolism Research; Pharmacology & Drug Discovery

Images & Validation

• Tested Applications: WB
• Dilution Range: Western blot: 0.5-2ug/ml, Immunohistochemistry (FFPE): 5ug/ml
• Reactivity: Human, Mouse
• Application Notes:
  Provided assay concentrations are suggestions only, LSD1 antibody titration may be required for optimal results.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: A recombinant protein fragment from the N-terminal region of human LSD1 was used as the immunogen for this antibody.
• Purification: Protein A affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.5 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin, 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Western blot testing of human samples with LSD1 antibody at 0.5 ug/ml. Expected molecular weight ~110 kDa.

Western blot testing of mouse samples with LSD1 antibody at 0.5 ug/ml. Expected molecular weight ~110 kDa.

IHC testing of FFPE human testis tissue with LSD1 antibody at 5 ug/ml. HIER: steam tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.

IHC testing of FFPE human breast tissue with LSD1 antibody at 5 ug/ml. HIER: steam tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.