MDH1 Antibody

SKU: orb345024

Description

Malate Dehydrogenase antibody

Research Area

Metabolism Research

Images & Validation

• Tested Applications: ELISA, IP, WB
• Dilution Range: ELISA: 1:5,000 - 1:20,000, IP: 1:100, WB: 1:500 - 1:5,000
• Reactivity: Porcine
• Application Notes:
  MALATE DEHYDROGENASE antibody has been tested by western blot and is suitable to be assayed against 1.0 ug of Malate Dehydrogenase [Pig Heart] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Sheep IgG [H&L] (Goat) and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:10,000 to 1:40,000 of the reconstitution concentration is suggested for this product.

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Key Properties

• Antibody Type: Primary Antibody
• Host: Sheep
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Malate Dehydrogenase [Pig Heart]
• Target: MDH1
• Purity: Anti-MALATE DEHYDROGENASE is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Sheep Serum as well as purified and partially purified Malate Dehydrogenase [Pig Heart]. Cross reactivity against Malate Dehydrogenase from other tissues and species may occur but have not been specifically determined.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

sheep anti-Malate Dehydrogenase Antibody, Malate dehydrogenase, cytoplasmic, Cytosolic malate dehydrogenase

Western Blot of Sheep anti-Malate Dehydrogenase antibody. Lane 1: Malate Dehydrogenase. Lane 2: None. Load: 50 ng per lane. Primary antibody: Malate Dehydrogenase antibody at 1:1000 for overnight at 4°C. Secondary antibody: Peroxidase sheep secondary antibody at 1:40000 for 30 min at RT. Block: orb348637 for 30 min at RT. Predicted/Observed size: 36 kDa, 36 kDa for Malate Dehydrogenase. Other band(s): None

Quick Database Links

Gene Symbol

MDH1

UniProt

P11708

RefSeq:P11708.4