MDH1 Antibody (HRP)

SKU: orb345039

Description

Malate Dehydrogenase antibody (Peroxidase)

Research Area

Metabolism Research

Images & Validation

• Tested Applications: ELISA, IHC, IP, WB
• Dilution Range: ELISA: 1:500 - 1:1,000, IHC: 1:500 - 1:2,500, IP: 1:100, WB: 1:1,000 - 1:5,000
• Reactivity: Porcine
• Application Notes:
  Anti-Malate Dehydrogenase Peroxidase Conjugated has been tested by western blot and is useful in ELISA and immunohistochemistry. Optimal titers should be obtained by researchers.

Key Properties

• Antibody Type: Primary Antibody
• Host: Sheep
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Malate Dehydrogenase [Pig Heart]
• Target: MDH1
• Purity: Malate Dehydrogenase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Sheep Serum as well as purified and partially purified Malate Dehydrogenase [Pig Heart]. Cross reactivity against Malate Dehydrogenase from other sources may occur but has not been specifically determined.
• Conjugation: HRP

Storage & Handling

• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

sheep anti-Malate Dehydrogenase HRP conjugated Antibody, peroxidase conjugated sheep anti-Malate Dehydrogenase Antibody, cytoplasmic, Cytosolic malate dehydrogenase

Western Blot of Sheep Anti-Malate Dehydrogenase Antibody Peroxidase Conjugated. Lane 1: Opal Prestained Molecular Weight Marker. Lane 2: Malate Dehydrogenase [0.05 µg]. Primary Antibody: Anti-Malate Dehydrogenase HRP at 1:1000 for 1 hr at RT. Secondary Antibody: none. Blocking Buffer: BlockOut (orb348644) for 30 mins at RT. Predicted MW: 30-35kDa. Exposure: 1sec.

Quick Database Links

Gene Symbol

MDH1

UniProt

P11708

RefSeq:P11708.4