MDH1 Antibody

SKU: orb750614

Description

MDH1 antibody

Research Area

Metabolism Research

Images & Validation

• Tested Applications: ELISA, IP, WB
• Dilution Range: ELISA: 1:5,000 - 1:20,000, IP: 1:100, WB: 1:500 - 1:5,000
• Reactivity: Porcine
• Application Notes:
  This product has been assayed against 1.0 ug of Malate Dehydrogenase [Pig Heart] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Sheep IgG [H&L] (Goat) and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:3,000 to 1:12,000 of the reconstitution concentration is suggested for this product.

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Key Properties

• Antibody Type: Primary Antibody
• Host: Sheep
• Clonality: Polyclonal
• Isotype: Antiserum
• Immunogen: Malate Dehydrogenase [Pig Heart]
• Target: MDH1
• Purity: This product was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-sheep serum, purified and partially purified Malate Dehydrogenase [Pig Heart]. Cross reactivity against Malate Dehydrogenase from other tissues and species may occur but have not been specifically determined.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Concentration: 80 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

sheep anti-Malate Dehydrogenase Antibody, Malate dehydrogenase cytoplasmic, Cytosolic malate dehydrogenase

Western Blot using Anti-Malate Dehydrogenase (Pig Heart) (SHEEP) Antibody. Two different molecular weight groups of isoforms were detected for MDH1 at about 36 kDa using Anti-MDH1 (pig heart) orb750614 at 1:1500, Fluorescent Cy-5 labelled secondary at 1:500 and for GDH at about 60 kDa using Anti-GDH (bovine liver) orb750578 at 1:1500, anti-rabbit secondary at 1:500. Light intensities revealed unchanged amount of total soluble MDH1 but increased amount of total soluble GDH in AD when compared to controls. Values of light intensities are given as mean ± S.E.M. *p ≤ 0.05.

Quick Database Links

Gene Symbol

MDH1

UniProt

P11708

RefSeq:P11708.4