MAPT Rabbit Polyclonal Antibody

SKU: orb580279

Description

MAPT Rabbit Polyclonal Antibody is an unconjugated, affinity purified antibody for the detection of MAPT. It is suitable for IHC, WB applications. The immunogen is a synthetic peptide directed towards the middle region of human MAPT. This antibody reacts with Human, Mouse samples and is predicted to react with Porcine, Rat. It is supplied as a liquid in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Research Area

Signal Transduction

Images & Validation

• Tested Applications: IHC, WB
• Reactivity: Human, Mouse
• Predicted Reactivity: Porcine, Rat

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human MAPT
• Target: MAPT
• Protein Sequence: Synthetic peptide located within the following region: RGAAPPGQKGQANATRIPAKTPPAPKTPPSSGEPPKSGDRSGYSSPGSPG
• Molecular Weight: 79 kDa
• Purification: Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Concentration: 0.5 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

TAU, MSTD, PPND, DDPAC, MAPTL, MTBT1, MTBT2, tau-40, FTDP-17, PPP1R103

25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 3 ug/ml of the antibody was used in this experiment. The peptide sequence is present in a 45 kDa isoform. The protein may be modified by acetylation, glycosylation, and phosphorylation.

Sample Type: A: Transgenic hTau P301L mouse cortex, B: Tau-Ko mouse cortex, C: WT mouse cortex, Primary Antibody dilution: 1:1000, Secondary Antibody: Anti-rabbit-biotin / Avidin-HRP, Secondary Antibody dilution: 1:1000 / 1:2000, Color/Signal Descriptions: Dark grey: MAPT, Gene Name: MAPT.

WB Suggested Anti-MAPT Antibody, Positive Control: Lane 1: 10 ug N2A expressed human Tau-C, Lane 2: 10 ug N2A expressed human Tau-E, Lane 3: 10 ug N2A expressed human Tau-F, Lane 4: 10 ug N2A expressed human Tau-B, Lane 5: 10 ug N2A expressed human Fetal-Tau, Lane 6: 10 ug N2A expressed human Tau-D, Lane 7: 10 ug N2A expressed human much Fetal-Tau, Lane 8: 10 ug N2A lysate, Primary Antibody Dilution: 1:2000, Secondary Antibody: Donkey anti-rabbit-HRP, Secondry Antibody Dilution: 1:10000.

WB Suggested Anti-MAPT Antibody Titration: 0.2-1 ug/ml, Positive Control: HepG2 cell lysate.

Quick Database Links

Gene Symbol

MAPT

UniProt

P10636

RefSeq (Protein):NP_005901