MICA Antibody

SKU: orb1535982

Description

MICA Antibody

Images & Validation

• Tested Applications: ELISA, IF, IHC, IHC-P, WB
• Dilution Range: ELISA, IF, IHC, IHC-P (5 µg/ml), WB (0.5 - 1 µg/ml)
• Reactivity: Human, Mouse
• Application Notes:
  Further information: MICA antibody can be used for detection of MICA by Western blot at 0.5 - 2 ug/mL. Antibody can also be used for immunohistochemistry starting at 10 ug/mL. For immunofluorescence start at 20 ug/mL. Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Western Blot: Predicted: 42 kDa Observed: 46 kDa

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Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: A 16 AA synthetic peptide near the C-Terminus of human MICA. The immunogen is located within the last 50 AA of MICA.
• Target: MICA
• Purification: Peptide Column Affinity Chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS containing 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

MICA, HLA class I antigen, MIC-A, PERB11.1

Immunofluorescence of MICA in Human Colon cells with MICA antibody at 20 ug/ml.

Western blot of MICA in A-20 cell lysate with MICA antibody at (A) 0.5, (B) 1 and (C) 2 ug/ml.

Quick Database Links

Gene Symbol

MICA