Mouse IgG (H&L) Antibody Pre-adsorbed

SKU: orb347626

Description

Mouse IgG (H&L) antibody

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Reactivity: Mouse
• Application Notes:
  Suitable for immunoblotting (western or dot blot), ELISA, electron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot consistency.

Key Properties

• Antibody Type: Secondary Antibody
• Host: Donkey
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Anti-Mouse IgG whole molecule was produced by repeated immunization with Mouse IgG whole molecule in donkey.
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum, Mouse IgG and Mouse Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Rabbit, Rat and Sheep Serum Proteins. Specificity was confirmed using ELISA at less than 1.0% cross reactivity against IgG proteins purified from the species listed above.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Donkey anti-Mouse IgG Antibody, Donkey anti-Mouse IgG pre-adsorbed Antibody

Western blot assay of L1 and SEMA 3A protein expressions at the proximal end (A) and (B) distal end of injured sensory and motor nerves. (A) Bands of L1 and SEMA 3A protein expressions at the proximal end of the injured nerves. (B) Bands of L1 and SEMA 3A protein expressions at the distal end of injured nerves. Primary antibody mouse monoclonal anti-neural cell adhesion molecule L1 (1:500), rabbit polyclonal anti-semaphorin 3A (1:1000), and mouse monoclonal to β-actin (1:10000) at 4°C overnight. Secondary antibody anti-rabbit IgG (H&L) (DONKEY) antibody (1:5000) or anti-mouse IgG (H&L) (DONKEY) antibody (1:5000) at room temperature for 1.5 hours. Experiments were performed in triplicate. MN: Motor nerves; SN: sensory nerves; L1: nerve cell adhesion molecule L1; SEMA 3A: semaphorin 3A.